Erros no preparo e na administração de medicamentos intravenosos / Errores en la preparación y administración de medicamentos intravenosos / Errors in the preparation and administration of intravenous drugs

Objetivo identificar os erros no preparo e na administração de medicamentos intravenosos. Método estudo observacional e descritivo realizado de 694 doses de medicamentos intravenosos realizadas pelos profissionais de enfermagem em pacientes adultos nas unidades de Emergência, Internamento e Unidade de Terapia Intensiva de um hospital de pequeno porte do recôncavo da Bahia, Brasil. Os dados foram coletados em março e abril de 2019, por meio da observação não participante, tendo como instrumento dois roteiros de observação. As variáveis descritivas foram analisadas por meio da distribuição de frequência absoluta e relativa. Resultados destacou-se os erros de técnica com observação da ocorrência de 60% de erros de técnica do preparo e 75% de erros de técnica de administração dos medicamentos intravenosos. Conclusão Tanto no preparo quanto na administração dos medicamentos intravenosos, foram identificados erros de técnica e de horário, apontando a necessidade de implementação de estratégias voltadas para a segurança do paciente.

Objetivo identificar errores en la preparación y administración de medicaciones intravenosas. Método estudio observacional y descriptivo, realizado con 694 dosis de medicaciones intravenosas realizadas por profesionales de enfermería en pacientes adultos en las Unidades de Emergencia, Internación y Cuidados Intensivos de un pequeño hospital en el recóncavo de Bahía, Brasil. Los datos fueron recolectados en los meses de marzo y abril de 2019, mediante observación no participante, utilizando dos guiones de observación como instrumento. Las variables descriptivas se analizaron mediante la distribución de frecuencia absoluta y relativa. Resultados se observaron errores técnicos, con ocurrencia del 60% de errores de técnica de preparación y 75% de errores de técnica de administración de fármacos intravenosos. Conclusión tanto en la preparación como en la administración de medicamentos por vía intravenosa se identificaron errores en la técnica y en el tiempo, señalando la necesidad de implementar estrategias orientadas a la seguridad del paciente.

Objective to identify errors in the preparation and administration of intravenous drugs. Method observational and descriptive study. 694 doses of intravenous medications performed by nursing professionals in adult patients were observed in the Emergency, Inpatient and Intensive Care Units of a small hospital in the recôncavo of Bahia, Brazil. Data were collected in March and April 2019, through non-participant observation, using two observation scripts as instruments. Descriptive variables were analyzed using the absolute and relative frequency distribution. Results the occurrence of 60% of errors in the preparation technique and 75% of errors in the technique of administering intravenous drugs was highlighted. Conclusion both in the preparation and administration of intravenous medications, errors in technique and time were identified, pointing out the need to implement strategies aimed at patient safety.

FGF2 Antiproliferative Stimulation Induces Proteomic Dynamic Changes and High Expression of FOSB and JUNB in K-Ras-Driven Mouse Tumor Cells.

Fibroblast growth factor 2 (FGF2) is a well-known cell proliferation promoter; however, it can also induce cell cycle arrest. To gain insight into the molecular mechanisms of this antiproliferative effect, for the first time, the early systemic proteomic differences induced by this growth factor in a K-Ras-driven mouse tumor cell line using a quantitative proteomics approach are investigated. More than 2900 proteins are quantified, indicating that terms associated with metabolism, RNA processing, replication, and transcription are enriched among proteins differentially expressed upon FGF2 stimulation. Proteomic trend dynamics indicate that, for proteins mainly associated with DNA replication and carbohydrate metabolism, an FGF2 stimulus delays their abundance changes, whereas FGF2 stimulation accelerates other metabolic programs. Transcription regulatory network analysis indicates master regulators of FGF2 stimulation, including two critical transcription factors, FOSB and JUNB. Their expression dynamics, both in the Y1 cell line (a murine model of adenocarcinoma cells) and in two other human cell lines (SK-N-MC and UM-UC-3) also susceptible to FGF2 antiproliferative effects, are investigated. Both protein expression levels depend on fibroblast growth factor receptor (FGFR) and src signaling. JUNB and FOSB knockdown do not rescue cells from the growth arrest induced by FGF2; however, FOSB knockdown rescue cells from DNA replication delay, indicating that FOSB expression underlies one of the FGF2 antiproliferative effects, namely, S-phase progression delay.